Wide spectrum of molecular and cellular biology, virology and immunology techniques including gene cloning, site-directed, deletion and insertion mutagenesis, gene expression, gene arrays, differential gene expression, yeast-two hybrid assays, promoter studies, cell proliferation and apoptosis assays, development of virus vectors and vaccines and T cell assays.
Smith, C.C., Yu, Y. X., Kulka, M., Aurelian, L. A novel human gene similar to the PK coding domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) codes for a serine-threonine PK and is expressed in melanoma cells. J. Biol. Chem. 275:25690-25699, 2000
Smith, C.C., Kulka, M., Aurelian, L. Modified adenovirus penton base protein (UTARVE) as a non-replicating vector for delivery of antisense oligonucleotides with antiviral and/or antineoplastic activity. Int. J. Oncol. 17:841-850, 2000
Smith, C. C., Nelson, J., Gober, M., Aurelian, L., Goswami, B. B. Binding of the GTP activating protein RAS-GAP by the PK domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10 PK) and activation of the RAS/MEK/MAPK mitogenic pathway are required for timely onset of HSV-2 growth. J. Virol. 74:10417-10429, 2000
Wachsman, M., Kulka, M., Smith, C.C. and Aurelian, L. A growth and latency defective herpes simplex virus type 2 mutant (ICP10DPK) has prophylactic and therapeutic protective activity in guinea pigs. Vaccine 19: 1879-1890, 2001.
Aurelian, L., Smith, C. C., Winchurch, R., Kulka, M., Zaccaro, L., Gyotoku, T, Chrest, F. J. Burnett, J. W. A novel gene expressed in human keratinocytes with long term growth potential is required for cell growth. J. Invest. Dermatol. 116:286-295, 2001
Perkins, D., Pereira, E. F. R., Gober, M. D., Yarowsky, P.J., Aurelian, L. The herpes simplex virus type 2 R1 protein kinase (ICP10 PK) blocks apoptosis in hippocampal neurons involving activation of the MEK/MAPK signaling pathway. J. Virol. 76:1435-1449, 2002
Perkins, D., Yu, Y., Bambrick, L. L., Yarowsky, P. J. Expression of Herpes simplex virus type 2 protein ICP10PK rescues neurons from apoptosis due to serum deprivation and genetic defects. Exp. Neurol. 174:118-122, 2002
Aurelian, L. HSV-Induced apoptosis in herpes encephalitis. Current Topics in Microbiology and Immunology 289: 79-112, 2005
Gober, M.D., Wales, S.Q., and Aurelian, L. Herpes simplex virus type 2 encodes a heat shock protein homologue with apoptosis regulatory functions. Frontiers in Bioscience 10, 2788-2803, 2005
Gober, M.D., Depre, C., Aurelian, L. Correspondence regarding M.V. Kim et al. "Some properties of the human small heat shock protein Hsp22 (H11 or HspB8). Biochem. Biophys. Res. Comm. 321:267-268, 2004
Gober, M.D., Wales, S.Q., Hunter, J.C., Sharma, B.K., Aurelian, L. Stress upregulates neuronal expression of the HSV-2 large subunit of ribonucleotide reductase (R1; ICP10) by activating AP-1. J. Neurovirol. 11:1-8, 2005
Ono, F., Sharma, B.K., Smith, C.C. Burnett, J.W., Aurelian, L. CD34+ cells in the peripheral blood transport herpes simplex virus (HSV) DNA fragments to the skin of patients with erythema multiforme (HAEM). J. Invest Dermatol. 124: 1215-1224, 2005
Laing, J.M., Gober, M.D., Golembewski, E.K., Thompson, S. M., Gyure, K.A., Yarowski, P.J. and Aurelian, L. Intranasal administration of the growth compromised HSV-2 vector deltaRR prevents kainate induced seizures and neuronal loss in rats and mice. Mol Therapy. In press, 2006
Sharma, B.K., Smith, C.C., Rucker, D., Laing, J.M., Burnett, J.W., Aurelian, L Aberrant DNA methylation silences the novel heat shock protein H11 in melanoma, but not benign melanocytic lesions. Dermatology, In press, 2006